Journal: Stem cells (Dayton, Ohio)

Article Title: Nuclear fusion-independent smooth muscle differentiation of human adipose-derived stem cells induced by a smooth muscle environment.

doi: 10.1002/stem.1023

Figure Lengend Snippet: Figure 6. Significant upregulation of human HGF in conditioned medium was confirmed by ELISA. HGF promotes smooth muscle differentia- tion of human adipose-derived stem cell (hASC) in a dose-dependent manner. (A): Increase of HGF from undetected (time 0) to more than 1000 pg/ml at 6 weeks differentiation. (B): At the lower concentrations, there was a dose-dependent induction of alpha smooth muscle actin (ASMA) in hASC exposed to HGF. Higher induction was seen at concentrations between 1 and 5 ng/ml of HGF at 6 weeks. All groups are statistically significant when compared to DMEM (p < .05). (C): Immunofluorescence staining confirmed fluorescence-activated cell sorter data. Double fluo- rescence staining visualizing the nuclei (blue) and ASMA (green). Undifferentiated hASCs before injection show 2%–3% baseline ASMA expres- sion (1). After 6 weeks in SMIM medium, there is an increase in the expression of ASMA (2). Further increase in ASMA expression is seen when cells are exposed to 5 ng/ml HGF for 6 weeks (3) (scale bar ¼ 100 lm). Abbreviations: DMEM, Dulbecco’s modified Eagle’s medium; FBS, fetal bovine serum; HGF, hepatocyte growth factor; SMIM, smooth muscle induction medium.

Article Snippet: Test and control media (DMEM or SMIM media exposed to hASC at time 0) were collected and examined for hepatocyte growth factor (HGF) levels using the Quantikine Human HGF ELISA kit (R&D Systems, Minneapolis, MN, www.rndsystems. com) according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Staining, Injection, Expressing

Journal: ACS nano

Article Title: A Biomimetic Multifunctional Nanoframework for Symptom Relief and Restorative Treatment of Acute Liver Failure.

doi: 10.1021/acsnano.4c00173

Figure Lengend Snippet: Figure 5. Effects of RMBN on hepatic inflammation in LPS/GalN-induced murine models of ALF. (A) Levels of TNF-α, IL-6, IL-1β, MCP-1, and IL-17A involved in liver inflammation were analyzed by ELISA in the liver (n = 7 mice). ###P < 0.005 (light blue), Model vs CON groups. *P < 0.05, **P < 0.01, ***P < 0.005, and nsP > 0.05 (orange), compared with the RMBN group. ∧∧∧P < 0.005 (purple blue), RMBN vs Model groups. (B, C) Immunoblot analysis of factors in NLRP3-reated signaling pathways include NLRP3, IL-18, ASC, pro-IL-1β, and IL- 1β in the liver. (D) Heatmap of the differential expressed genes related to the NLRP3 inflammasome activation in the livers of mimic ALF mice with or without the RMBN (n = 4). RMBN + LPS/GalN vs LPS/GalN groups. Colors represent log2-transformed count (log2) change after normalization. (E, F) Immunoblot analysis of factors in NLRP3-reated signaling pathways include NLRP3, IL-18, ASC, pro-IL-1β, and IL-1β in the liver. (G) Representative immunofluorescence staining of CD206+ (red) and iNOS (green) in mouse liver sections. Scale bar = 50 μm. Data are expressed as means ± SD (n = 3−7). *P < 0.05, **P < 0.01, ***P < 0.005, and nsP > 0.05 compared with the vehicle group.

Article Snippet: Mouse CXCL12 ELISA Kit, Mouse IGF-1 ELISA Kit, and Mouse HGF ELISA Kit were purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Protein-Protein interactions, Activation Assay, Transformation Assay, Immunofluorescence, Staining

Journal: Antioxidants

Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction

doi: 10.3390/antiox10111741

Figure Lengend Snippet: Pre- and post-extraction OPG, RANKL, HGF, TNF-α, IL-18, MMP-9, TOS—total oxidant status, TAC—total antioxidant capacity, and HbA1c values in control and diabetes mellitus patients.

Article Snippet: A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ].

Techniques: Control

Journal: Antioxidants

Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction

doi: 10.3390/antiox10111741

Figure Lengend Snippet: ROC curve. Prediction of salivary markers in the determinism of HbA1c > 6.5%.

Article Snippet: A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ].

Techniques:

Journal: Antioxidants

Article Title: Correlations between Salivary Immuno-Biochemical Markers and HbA1c in Type 2 Diabetes Subjects before and after Dental Extraction

doi: 10.3390/antiox10111741

Figure Lengend Snippet: Post-extraction correlation of HbA1c with salivary markers (OPG, RANKL, OPG/RANKL, HGF, TNF-α, MMP-9, IL-18, TOS and TAC).

Article Snippet: A number of parameters were determined in saliva, using the ELISA immunenzymatic method: OPG (MyBioSource, San Diego, CA, United States, # MBS175881), RANKL (MyBioSource, San Diego, CA, United States, # MBS268235), HGF (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0084), TNF-α (Elabscience, Houston, Texas, United States, Catalog no. E-EL-H0109), MMP-9 (Abcam, CA, United Kingdom, ab246539), IL-18 (Abcam, Cambridge, United Kingdom, ab215539), and the spectrophotometric method determined TAC (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey) and TOS (Rel Assay Diagnostics Kit, Mega Tıp, Gaziantep, Turkey), according to the method described by Erel in 2005 [ ].

Techniques: Extraction

Journal: American Journal of Translational Research

Article Title: Portal modulation effects of terlipressin on liver regeneration and survival in a porcine model subjected to 90% hepatectomy

doi:

Figure Lengend Snippet: Postoperative change in serum levels of interleukin (IL)-6 (A), hepatocyte growth factor (HGF) (B), and endothelin-1 (ET-1) (C) evaluated using enzyme-linked immunosorbent assay. Data are expressed as the median, with the 25-75% percentiles in boxes and the 5-95% percentiles as whiskers. *P < 0.05 vs. control group at the same time point.

Article Snippet: Interleukin 6 (IL-6), hepatocyte growth factor (HGF), and endothelin-1 (ET-1) serum levels were measured using commercially available ELISA kits (IL-6; Porcine IL-6 Quantikine ELISA Kit, P6000B, R&D systems, USA) (HGF; Pig hepatocyte growth factor, HGF ELISA Kit, CSB-E06795p, CUSABIO, China) (ET-1; Endothelin-1 Quantikine ELISA Kit, DET100, R&D systems, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Scientific Reports

Article Title: Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels

doi: 10.1038/s41598-022-26041-0

Figure Lengend Snippet: Secretion of growth factors by adipose-derived stem cells (ASCs) and the formation of networks by co-culture with endothelial cells (ECs) or lymphatic endothelial cells (LECs). ( a ) The levels of vascular endothelial growth factor-C (VEGF-C), VEGF-A, hepatocyte growth factor (HGF) and basic fibroblast growth factor (bFGF) in the medium bathing cultured ASCs were measured using enzyme-linked immunosorbent assays. HGF and VEGF-A were both secreted by ASCs, whereas the levels of secreted VEGF-C and b-FGF were much lower. ( b ) Green fluorescent protein (GFP)-expressing ECs and ASCs co-cultured at a ratio of 1:4. Networks of vascular ECs were observed, and the edges of the vascular EC network were sharp (arrows). ( c ) GFP-LECs and ASCs co-cultured at a ratio of 1:4. The LECs formed networks, but in contrast to vascular ECs, rounded/bulging structures were observed at the edges of the LEC network (arrowheads). ( d ) Merge image of ( c ) and phase-contrast image. ASCs shows random pattern with no specific character which do not form own network or support LEC network directly.

Article Snippet: The concentrations of VEGF-C, VEGF-A, HGF, and bFGF were measured by enzyme-linked immunosorbent assay (ELISA) using a Human VEGF-C ELISA Kit (P49767, RayBiotech, USA), LBIS Human VEGF ELISA Kit (631-40831, Fujifilm Wako, Japan), AuthentiKine Human HGF ELISA Kit (KE00168, Proteintech, USA) and Human bFGF ELISA Kit (P09038, RayBiotech, USA) in accordance with the manufacturers’ instructions.

Techniques: Derivative Assay, Co-Culture Assay, Cell Culture, Expressing